| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051519 |
| Chromosome: | chromosome 6 |
| Location: | 47448 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g248950 | (1 of 3) K11855 - ubiquitin carboxyl-terminal hydrolase 36/42 [EC:3.4.19.12] (USP36_42) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCTGTAACCCGCCAAAACAGCTGGTGCCCCGCCTGTGAGCTTTCCGA |
| Internal bar code: | CTGACGAGGGTTAGCATTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2278 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACATGCGGCACACAGGAT |
| Suggested primer 2: | ATTCAGCTGAGGATGTGGGC |