| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051523 |
| Chromosome: | chromosome 3 |
| Location: | 4244206 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173950 | (1 of 3) PF08495 - FIST N domain (FIST) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCCCGCCCACAGCCGCCCACGGAAGGGGCCTAGCAAGCACTGCAT |
| Internal bar code: | TGTAGGACGCAGCCGGATTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3406 |
| LEAP-Seq percent confirming: | 77.3585 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCACAGCCGTTTAGTT |
| Suggested primer 2: | GCGAATGCTCACGGATGTTC |