| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051528 |
| Chromosome: | chromosome 9 |
| Location: | 3689892 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397253 | (1 of 1) PTHR11748//PTHR11748:SF75 - D-LACTATE DEHYDROGENASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGACGTAGGGCCAAGCTGCATGTTCGTGTAGCTGTCCATATTGTTTC |
| Internal bar code: | CCATGAATATTAGTGTCATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1645 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGCATCTAAACCCCCTT |
| Suggested primer 2: | CTGAGGCTATCCGAGTGCTG |