| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051532 |
| Chromosome: | chromosome 1 |
| Location: | 7589155 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800139 | (1 of 1) IPR026741//IPR026937//IPR027417 - Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGGCAGGGGGACTCTTTCACGCCTCCGACTCTCCCAGCCGCCCCTCC |
| Internal bar code: | ATGCCTATTGAGAAGGGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1314 |
| LEAP-Seq percent confirming: | 71.0526 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGAGGAGGAATTGGTGG |
| Suggested primer 2: | CCTCCCAACACTTCCATCCC |