| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051533 |
| Chromosome: | chromosome 2 |
| Location: | 4139489 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103750 | intron | ||
| lncRNA_TCONS_00041505 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGATTGAGTCACTAGGCTTGAGTTTATGCAGACACGCCCTGGCAACCG |
| Internal bar code: | TGTGGCTATACGCGGACAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4202 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTATCCTTGCAACACCA |
| Suggested primer 2: | GTTCCACTTTTCAGCGCGTT |