Insertion junction: CLIP2.051534_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051534
Chromosome:chromosome 5
Location:2785027
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g240900 (1 of 1) IPR000001//IPR024079 - Kringle // Metallopeptidase, catalytic domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGACAATTGGAGCTGCTTGTCCTCCATTCCACACGACCTCCAATCTTG
Internal bar code:GAAGTACTGTTCAATGTGACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:374
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCCAAACTCCAAGCCAAA
Suggested primer 2:TGTCCATCTACAACGACGGC