| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051540 |
| Chromosome: | chromosome 2 |
| Location: | 3729131 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100633 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGGCGCTGGTGGCGGTGCTGGCGGGCGCGTGGGGGGCCGCTTCCGGC |
| Internal bar code: | TATGTGGTGTCGTGTGAATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5083 |
| LEAP-Seq percent confirming: | 99.0476 |
| LEAP-Seq n confirming: | 208 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 210 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAACCAGGGTGCAAGAG |
| Suggested primer 2: | CGCTGTCGCTTTCCTATTGC |