| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051541 |
| Chromosome: | chromosome 4 |
| Location: | 2926329 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224600 | (1 of 1) IPR000253//IPR011598 - Forkhead-associated (FHA) domain // Myc-type, basic helix-loop-helix (bHLH) domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCTGACCCATGGCACCCTCTACGGCGCCCGCTTAGCCCACTGCTGC |
| Internal bar code: | TCAACCTATAGAGGGTATGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3498 |
| LEAP-Seq percent confirming: | 93.2203 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 118 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCGCCACATCAAACATG |
| Suggested primer 2: | ACCCATGACGTATCACAGCG |