| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.051547 |
| Chromosome: | chromosome 14 |
| Location: | 3110799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628400 | TSA3 | (1 of 3) 6.1.1.7 - Alanine--tRNA ligase / Alanyl-tRNA synthetase; Alanyl-tRNA-synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAACACAAGTCATGTTTGCACACACAATATAGACCATTTCGCGGCCACG |
| Internal bar code: | TGAGACTAAATATATATAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1760 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATACACACCGACTTGCCT |
| Suggested primer 2: | TGCGAGGTAGAAGCACGTTT |