Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.051547 |
Chromosome: | chromosome 14 |
Location: | 3110803 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g628400 | TSA3 | (1 of 3) 6.1.1.7 - Alanine--tRNA ligase / Alanyl-tRNA synthetase; Alanyl-tRNA-synthetase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCATCGCGCATTGTGCCCTGCAGGTTCTGGAGCTGGAGGCGCGTGCC |
Internal bar code: | AATATAGTAAGCAAGCGGTTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3785 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 18 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCGAGGTAGAAGCACGTTT |
Suggested primer 2: | CCATACACACCGACTTGCCT |