| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051550 |
| Chromosome: | chromosome 3 |
| Location: | 4735176 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177550 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAAGCAAAGAGAGGTTGGTACGGGCGGAGGCGCGGTGAATGATCTGT |
| Internal bar code: | GCGTCTCCTGGGTGTGGTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2757 |
| LEAP-Seq percent confirming: | 57.4074 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACCTGACCGTGAACTGG |
| Suggested primer 2: | GCTTTCGGGTGATGATGGGA |