| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051560 |
| Chromosome: | chromosome 8 |
| Location: | 2913572 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374400 | (1 of 1) PTHR19920//PTHR19920:SF0 - WD40 PROTEIN CIAO1 // CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED | 5'UTR | |
| Cre08.g374436 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACAGCCAACAGCGCTCCCAACGGGCAACGTTCTAGCCACCAACACTG |
| Internal bar code: | TTTGTCATGGTGTGTGGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2561 |
| LEAP-Seq percent confirming: | 86.2069 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATACGCCCACGGATGAACG |
| Suggested primer 2: | CAGCTGATTGATGACACGCG |