| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051585 |
| Chromosome: | chromosome 7 |
| Location: | 5116064 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348200 | SPH3 | Sucrose-phosphate phosphatase; (1 of 1) PTHR12526:SF2 - SUCROSE-PHOSPHATASE 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACATTACACTCTGTCGTGTGTGCGCCACACACCCGCGTAAATCAGGCC |
| Internal bar code: | CGCGAGTTTGGCCTGCCTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1403 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCAGGGGCATACACAAG |
| Suggested primer 2: | GGATCAGCAGGTCCAAAGCT |