| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051597 |
| Chromosome: | chromosome 7 |
| Location: | 5175823 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348700 | (1 of 1) PF09429 - WW domain binding protein 11 (Wbp11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTCCGCCACCGTGGCGAGGAATTCTAGGTATTTCTGGTCGGTCGAGA |
| Internal bar code: | GCCTGAGCTCATGCTATCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2045 |
| LEAP-Seq percent confirming: | 65.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCACATGATGAGTGAGT |
| Suggested primer 2: | CCACGATAACAGCCAGGGTT |