Insertion junction: CLIP2.051598_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051598
Chromosome:chromosome 7
Location:5856658
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353750 MMP10 Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAATATGGATTCCCGCGACGTTCTCTGCCTGCGGCCCCCAACGCGCGCG
Internal bar code:ATTGCGAGGCGCTTCGGCCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1313
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTACTCAACTGCGCACGA
Suggested primer 2:CTTCCCATTCTTGTCCGCCT