| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.051622 |
| Chromosome: | chromosome 3 |
| Location: | 5903997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188750 | HEL21 | (1 of 1) K13177 - ATP-dependent RNA helicase DDX1 [EC:3.6.4.13] (DDX1); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGTCCGTGTATGTGGGGTATCAAACCCAAGCCGCAATCCTACAGGCA |
| Internal bar code: | GCGTTTCTCTCACTAAATGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1260 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCAACATGACCCTGCCC |
| Suggested primer 2: | GCCACGACTGGAAAGGAAGA |