| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051626 |
| Chromosome: | chromosome 1 |
| Location: | 2733476 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016542 | (1 of 1) IPR000104//IPR029063 - Antifreeze protein, type I // S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGGTCCCGGCATGCCCGACACGCGGTGGCTTGTCCAGCTGCCTGAC |
| Internal bar code: | GTGGCTGTCGCTGCAATTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4458 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGTGGTACTCGGAGTCG |
| Suggested primer 2: | CTGATGCTGCCGGTAATTGC |