| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051660 |
| Chromosome: | chromosome 12 |
| Location: | 5223519 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCAGACAGGGGACCCCAACACGCAGCTGTTAGGATGGCCCGCCCCGCC |
| Internal bar code: | TCCAGGCTAGAGCGGTATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3805 |
| LEAP-Seq percent confirming: | 96.5909 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGTGACACCAGCTTGC |
| Suggested primer 2: | CCAGAACCCCAAGCCCTTAG |