Insertion junction: CLIP2.051686_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051686
Chromosome:chromosome 6
Location:3736049
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278162 PHC3 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGTTGCGGTCCAACTCATCCCTAGATATGTAACCTAGCCACCCCGTG
Internal bar code:CGTTTTTTGGGGTTGATTCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:361
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGTCAACGTACAGCTTCA
Suggested primer 2:GGCTTCCAAGTTTCTGCTGC