Insertion junction: CLIP2.051706_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.051706
Chromosome:chromosome 12
Location:2884987
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g502901 (1 of 3) PF08263//PF13855 - Leucine rich repeat N-terminal domain (LRRNT_2) // Leucine rich repeat (LRR_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAATACCGGTAATCGCACTCCCTAGTATCATGTGTGACCTTTGGTGTT
Internal bar code:GGGTCTATTTACTCGAATGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2693
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCATAGGCCCCTGAACAGTT
Suggested primer 2:TACGACCCGCATTATCAGGC