| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051707 |
| Chromosome: | chromosome 16 |
| Location: | 5537278 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTACTTCAACGCGTTCGCCCGTGTCGCGTTGTACTTACTGCTCTCCTC |
| Internal bar code: | ACAGGTTACATTAAAGCTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3338 |
| LEAP-Seq percent confirming: | 94.2308 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCCTCTAATGCCGTGCG |
| Suggested primer 2: | CATGCGTGTCCTTTGTGCAA |