| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051721 |
| Chromosome: | chromosome 3 |
| Location: | 2453246 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159016 | (1 of 13) 2.7.11.1//2.7.11.25 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Mitogen-activated protein kinase kinase kinase / MLTK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCTCACCTCCACCTCCTGCGGGAGTAGCCGCAACGGAAGAGAGCAGG |
| Internal bar code: | AAGTTGCACCCTGGAATGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3841 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGGAGCAGAACGTCCAA |
| Suggested primer 2: | GCTGTGGCTGTTGCATTTCA |