| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.051733 |
| Chromosome: | chromosome 1 |
| Location: | 5229187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036600 | EXN3 | (1 of 2) K14570 - RNA exonuclease 1 (REX1, REXO1, RNH70); Exoribonuclease II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCGCAGTAGGCGCGCACTCTGAGACACCCACACTGCGCCCGCACTAA |
| Internal bar code: | GCTCCCATACCATGACTTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGACAAGGACCTCGCTG |
| Suggested primer 2: | TCCGGCCTAAGAAGCAAAGG |