Insertion junction: CLIP2.051736_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.051736
Chromosome:chromosome 2
Location:2857349
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAATGAGATGCAACGCGAGTACAGGCATCTGCACGGGACTGGGGCTCC
Internal bar code:TTATGTACAGGATATTCACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1496
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACTACTGTGGCGCATCA
Suggested primer 2:CGCGGCTGAATGTTAGAAGC