| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051745 |
| Chromosome: | chromosome 3 |
| Location: | 4101063 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172350 | (1 of 1) PF14736 - Protein N-terminal asparagine amidohydrolase (N_Asn_amidohyd) | 5'UTR | |
| Cre03.g172400 | (1 of 100) IPR001810 - F-box domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACAGTGTCGGGCCCCCTCGCATGGCGTTTGACCCTGGTGTGATGCAA |
| Internal bar code: | GCAAATATTAGGGTATCTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4781 |
| LEAP-Seq percent confirming: | 91.8367 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAGAGCGAGGGGATTCA |
| Suggested primer 2: | CGCTTCCCGTACGAGCTTAT |