Insertion junction: CLIP2.051752_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.051752
Chromosome:chromosome 13
Location:4984104
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g801583 (1 of 2) PF00665//PF07727//PF13976//PF14223 - Integrase core domain (rve) // Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_2) // GAG-pre-integrase domain (gag_pre-integrs) // gag-polypeptide of LTR copia-type (UBN2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCTGGCGTGAAGCCCAGCCCGCCCAGCTCCTCACTGAGCTTGCGGTA
Internal bar code:GCATGGTCTACCATCTTTCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2244
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGGCATAGACTACAGCG
Suggested primer 2:CGACTCCACAGAAGAGGCAG