| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.051756 |
| Chromosome: | plastome |
| Location: | 193462 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802334 | orf528 | unknown ORF | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTACTTTTCCAATACAAGAAAACAACTTGATGGAAGAAGCGCAATTTG |
| Internal bar code: | GACTGTAGTCTTCTGATATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGTTAAGCTCGAACCCC |
| Suggested primer 2: | TCCTCTTTGGGGTTGCACAG |