Insertion junction: CLIP2.051757_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.051757
Chromosome:chromosome 12
Location:6561970
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g538300 FAP247,LF5 Flagellar Associated Protein 247; (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATCCTCTGTAAGCATCCCCATGAAGGTCCGGCATCCGTTAGTGAGGG
Internal bar code:ATCGAGTATTTGTTTGTTTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1465
LEAP-Seq percent confirming:35.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGACTAGCACCCGAAGCC
Suggested primer 2:TGGATAGCTACAGGGGCAGT