| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.051771 |
| Chromosome: | chromosome 7 |
| Location: | 3487995 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335950 | (1 of 2) K10523 - speckle-type POZ protein (SPOP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATTCGAGCGGCTGACTGCAGCGGACAAGCAGGTCTCACCAACGTTTGT |
| Internal bar code: | TCCTTAGCTGTACGATTAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2794 |
| LEAP-Seq percent confirming: | 95.3488 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGAAGGACGCACGAGTA |
| Suggested primer 2: | TACATGTCGTTGTGGCGGAA |