Insertion junction: CLIP2.051792_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.051792
Chromosome:chromosome 2
Location:6785338
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387949 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGTGGCTGCGCGAGTGACGGCAGTGCTGCACTGGACGTGTCACCTGCC
Internal bar code:GTTGCGGGCGCTGTAAGTCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1807
LEAP-Seq percent confirming:45.7143
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGTCCAAGAAGATGGCA
Suggested primer 2:TCCTCTTTGTCGCCAGGAAC