| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051797 |
| Chromosome: | chromosome 12 |
| Location: | 4767535 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523150 | PFD5 | Prefoldin alpha subunit; (1 of 1) K04797 - prefoldin alpha subunit (pfdA, PFDN5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCAAGAAGCAAGCAGAAGGCAAAGCAGCTGGTTGGGGGCTTGGGGC |
| Internal bar code: | TGTTGGACGATTGAGGCAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4716 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGATGTCTCTAGCTGCGG |
| Suggested primer 2: | ACTTCCATCAGCCGCATGAA |