Insertion junction: CLIP2.051798_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.051798
Chromosome:chromosome 4
Location:2694176
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g223375 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTCGAGGAAAGCTGTGATGACGAAGGACTAGACGGCAGCGCAGCTTG
Internal bar code:TTCCTTTGACAGCATTATTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2243
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GTACAGAATCTGCAAGCGCG
Suggested primer 2:CTGCTGTTGACAACTGTGCC