| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051823 |
| Chromosome: | chromosome 10 |
| Location: | 6581159 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465700 | (1 of 1) PF01082//PF03712 - Copper type II ascorbate-dependent monooxygenase, N-terminal domain (Cu2_monooxygen) // Copper type II ascorbate-dependent monooxygenase, C-terminal domain (Cu2_monoox_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACCTACAACCATGCCCATACCCACACACCTACAACCACACCTACAA |
| Internal bar code: | CGATTAGTTATTCGGTATCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGTCCCTACCCTTTTC |
| Suggested primer 2: | GATCTATCGGTCAGCGCACA |