| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051830 |
| Chromosome: | chromosome 3 |
| Location: | 8982527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800413 | (1 of 1) PTHR10445 - GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGACCAGATGAAGCGGCCGCGGCTGGAGCGCGAGGAGCTGGAGGAGAT |
| Internal bar code: | TTATTGGCGATGTTGTGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 7.31707 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGTGTGAGGTTGTGGGG |
| Suggested primer 2: | GCAACGTCAACCCAACATCC |