| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.051848 |
| Chromosome: | chromosome 12 |
| Location: | 8563982 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548900 | ROC23,ROC112 | (1 of 2) PTHR23181//PTHR23181:SF7 - RHO GTPASE ACTIVATING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGGCTGCGAAGAAGGTTTCGGTATACCTCTCCTTCGGAGGAGCGACT |
| Internal bar code: | TGACGTGTATCTAGCTTAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACTCCACCTCATCGGCT |
| Suggested primer 2: | TCGGACGCAAAGACATGACA |