Insertion junction: CLIP2.051869_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.051869
Chromosome:chromosome 10
Location:637760
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421900 SRR27 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGATCAACCGCACCGATGCCTCTTGGGGCGCCGTACTTTCGGGGATTG
Internal bar code:GTTTTCGGAAGCGTCTGTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:838
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCACACTGGAAAGGTTG
Suggested primer 2:CGAGGCGCATTATTGTGGTG