| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051869 |
| Chromosome: | chromosome 10 |
| Location: | 637760 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421900 | SRR27 | Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCGAAATGCTGGTGTTGCGGAAGACGATGCTGCCGTAGTAATAACC |
| Internal bar code: | GTTTTCGGAAGCGTCTGTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1713 |
| LEAP-Seq percent confirming: | 65.2174 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGCGCATTATTGTGGTG |
| Suggested primer 2: | CACGCACACTGGAAAGGTTG |