Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.051878 |
Chromosome: | chromosome 14 |
Location: | 1244467 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g616000 | (1 of 35) IPR001763 - Rhodanese-like domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGTGAACACTGTGTGTGTGGACAGCTCGTGCCGCCTCCGCCGCCCA |
Internal bar code: | TAGCTTCGCCTCCGCCATAGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2557 |
LEAP-Seq percent confirming: | 87.0968 |
LEAP-Seq n confirming: | 27 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATGCACAGTCAAACACGCA |
Suggested primer 2: | GATGCGATTCGTGCAGGTTC |