Insertion junction: CLIP2.051880_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.051880
Chromosome:chromosome 16
Location:6788103
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g672250 MPA13 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCAGAGCATCCAGGCAACAGCTCTGGAAGATGGCGGGGTTCAGGACTA
Internal bar code:TCTCGGTAGCCAACTGGCTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1553
LEAP-Seq percent confirming:94.3396
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTACTGTAGTCTCGGG
Suggested primer 2:CCCACTTGCGTTCTCCAGAT