| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051895 |
| Chromosome: | chromosome 10 |
| Location: | 828653 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423300 | (1 of 1) 3.4.22.40 - Bleomycin hydrolase / Aminopeptidase C (Lactococcus lactis) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCTTACCGTATGACACACACACACTACGCACACACACCCGCCTGACA |
| Internal bar code: | CTTAGAACTGGTCCAGGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1557 |
| LEAP-Seq percent confirming: | 30.1887 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGTGTGTGTGGTGTGT |
| Suggested primer 2: | GAGAGGTGGTGGTCGTTGAG |