| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051905 |
| Chromosome: | chromosome 16 |
| Location: | 2853418 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662951 | (1 of 1) PF06985//PF12796//PF13637 - Heterokaryon incompatibility protein (HET) (HET) // Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGAGCTGGCGCTGGGGCATGCCCAAGCCCACCGTGTACCGGCCCGGC |
| Internal bar code: | GTGACTCAATATTGGCGGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1220 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCATGGGGGAGGCAAGTG |
| Suggested primer 2: | AAATCAGGCTGAGGACGGTG |