| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051913 |
| Chromosome: | chromosome 8 |
| Location: | 2027551 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g368750 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTTCATCCCCTCCACATGCTCATCATGCCTCACACTGTGTCTTAGCT |
| Internal bar code: | ACTACAAGTCTTTGCGGAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2090 |
| LEAP-Seq percent confirming: | 80.6452 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGACTGCAAAAGTGGCC |
| Suggested primer 2: | GCTATGGCGCTAATGGAGGT |