| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.051926 |
| Chromosome: | chromosome 3 |
| Location: | 1266220 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149900 | (1 of 1) IPR027369 - DinB/YfiT-like putative metal-dependent hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGGACCACGCGGTGAAGCTGCGGCTTGAGGCGGCGAGGCTGCGGCAG |
| Internal bar code: | GTCGGATCGGAGGCCAAGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3246 |
| LEAP-Seq percent confirming: | 59.5238 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATATGCTGGAGCGACTT |
| Suggested primer 2: | ATTGGGGAGCTCAGCAACTC |