| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.051928 |
| Chromosome: | chromosome 8 |
| Location: | 2931912 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374650 | DII3,TPR5,p44 | Subunit of axonemal inner dynein d with TPR domains; (1 of 1) PTHR10098:SF120 - TETRATRICOPEPTIDE REPEAT PROTEIN 29 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCGGTATGCCTGCGCCTCTGCGGCCTCCGCCTCCGCAATGAGGTTGT |
| Internal bar code: | GGCCTAGTGGGGTTTTGTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1063 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGCATGGCAACTTTGG |
| Suggested primer 2: | ACCCACCCCCTATTGCATTG |