Insertion junction: CLIP2.051949_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.051949
Chromosome:chromosome 3
Location:5872762
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g188400 RBO2,RBOL2 (1 of 2) 1.6.3.1 - NAD(P)H oxidase (H(2)O(2)-forming) / Thyroid oxidase 2; Respiratory burst oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGAGAGCATGCCGCACGCTATCCATATCACACATAACAAGGTCGGCGC
Internal bar code:TGGGCTTGAGTTGTGGCGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2598
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCAGCTGAGGATCGAGAC
Suggested primer 2:TTGCGCGCATTCTGTTTCAA