Insertion junction: CLIP2.051967_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.051967
Chromosome:chromosome 2
Location:4576598
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g107150 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTAAAGTTATGTGTGCCGAATACCTTGCTGGTACCTGTGTTACCCTA
Internal bar code:CAGGAAGGGGGTGGCTTCCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4058
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:132
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:132

Suggested primers for confirmation by PCR

Suggested primer 1:CACCAGCGCAATGATACACG
Suggested primer 2:CCTCCACACAGCAGTCACAT