| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051968 |
| Chromosome: | chromosome 13 |
| Location: | 4134865 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591200 | HTB38,HTB22 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGCTAAGAAGGCTGCCAAGGAGGGCGGCGACGGCGAGAAGGGCGACA |
| Internal bar code: | CAGGGTATCGAATTCCCTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2440 |
| LEAP-Seq percent confirming: | 88.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTAGCCCGAGGTGAACT |
| Suggested primer 2: | GGAGGCTCTTGTGACCTTCC |