Insertion junction: CLIP2.051983_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.051983
Chromosome:chromosome 16
Location:743138
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g690950 (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAAACGAATTGTTGTGGCGCTTTGATGCTGTGCCCGAGAGGCCCGACC
Internal bar code:TGTCGAACATGATACAACGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2000
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGCGTGCTTGAAAACCAC
Suggested primer 2:CCAGCTCCACACCTTCACAT