| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052012 |
| Chromosome: | chromosome 1 |
| Location: | 5125784 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035800 | IRK2 | (1 of 2) PTHR11767//PTHR11767:SF47 - INWARD RECTIFIER POTASSIUM CHANNEL // SUBFAMILY NOT NAMED; Inwardly-rectifying potassium channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTTGGAGGCGCGGCTGGGGGGGTAAGAGGGGAAGGGCGTAAGGGATT |
| Internal bar code: | GGACTCGGACCCATCCCTTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2503 |
| LEAP-Seq percent confirming: | 64.3836 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTGGCTTTGTTGCTTT |
| Suggested primer 2: | AACAGCGCGAAGAACAGGTA |