Insertion junction: CLIP2.052026_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.052026
Chromosome:chromosome 13
Location:2173174
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g577850 FKB20,FKB20B,FKB20-2 (1 of 1) PTHR10516:SF178 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP20-2, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACAACGCTGGCGTGCCGCCAGACATCTGCATACTGCCACACGGCGAT
Internal bar code:GGTGTTCGTGCGTCATCCGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2066
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACCTACCTCGACACGGA
Suggested primer 2:CAGTGCGAGGTCAGTGGATT